Restriction digests of the clone give the following sizes (kb): HindIII--6.0, 3.5; XbaI--9.5; BamHI--9.5. SOS recruitment system (SRS) is a genetic screening method to detect proteins interacting in the cytoplasm. It is based on membrane targeting with a myristoylation signal and SOS-based activation of Ras protein. The vector contains the human SOS gene with a polylinker on the N-terminus to attach "bait" gene for use in SOS Recruitment System. |
