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Size (kb): 6.00

Vector: pcos6EMBL (cosmid)

Promoters: Promoter none

Construction: pcos5EMBL, Pi

Marker(s):kanR

Construct size (kb): 6.0

Features: marker(s): kanR
promoter: none
replicon: R6K
terminator: none
enhancer: none

vector for constructing cDNA libraries

Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8.

IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.

One of a family of cosmid vectors (ATCC 37568-37571) containing a mutated R6K origin for increased copy number.

Has increased copy number over pcos5EMBL (ATCC 37570).

Temperature: 30.0°C