用轉(zhuǎn)座子miniTn10插入四硫酸鹽呼吸相關(guān)基因,然后培養(yǎng)篩選其中TR-菌株,最后用原噬菌體確定插入位點(diǎn)。

參考文獻(xiàn):Hensel M, Hinsley AP, Nikolaus T, Sawers G, Berks BC. The genetic basis of tetrathionate respiration in Salmonella typhimurium. Mol Microbiol. 1999 Apr;32(2):275-87.