菌株簡(jiǎn)介
HT115(DE3) 是一個(gè)RNAi Feeding 菌株，抗性是四環(huán)素Tet，可用37℃，LB，有氧的方式培養(yǎng)，主要用于線蟲(chóng)的干擾實(shí)驗(yàn)。 HT115 is an Rnase III-deficient E. coli strain with IPTG-inducible T7 Polymerase activity. To induce dsRNA production from these plasmids, the HT115 bacteria is grown on special RNAi NGM feeding plates that contain IPTG and the ampicillin analog carbenicillin. Carbenicillin is preferred over ampicillin because it tends to be more stable. Bacterial strain. Genotype: F-, mcrA, mcrB, IN(rrnD-rrnE)1, rnc14::Tn10(DE3 lysogen: lavUV5 promoter -T7 polymerase) (IPTG-inducible T7 polymerase) (RNAse III minus). This strain grows on LB or 2XYT plates. This strain is tetracycline esistant. Researchers using this strain should test for expression by transforming in one of the plasmids from the Fire Vector Kit (1999) (pLT76, e.g.) using standard CaCl2 transformation techniques. Biosafety Level: BSL-1. Bacteria-mediated RNAi--General outline:
