Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
The reported population doubling time decreases with increasing passages from approximately 55 hours at passage 70 to 30 hours at passage 90.
The cells will grow well (but at a slower rate) in medium containing decreased amounts of serum (5% bovine calf serum, 2.5% fetal bovine serum).
Gospodarowicz D, et al. Stimulation of corneal endothelial cell proliferations in vitro by fibroblast and epidermal growth factors. Exp. Eye Res. 25: 75-89, 1977. PubMed: 302215
Bethea CL, Kozak SL. Effect of extracellular matrix on PC 12 cell shape and dopamine processing. Mol. Cell. Endocrinol. 37: 319-327, 1984. PubMed: 6500171
