Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
The SHP-77 (Shadyside Hospital, Pittsburgh, PA) cell line is a biochemically stable continuously cultured cell line which has retained important features of SCLC.
This cell line is an unusual undifferentiated large cell variant of small cell lung carcinoma.
It has the morphology of a variant, but the biochemical properties of a classic SCLC.
Electron microscopy revealed the presence of gland formation and intracytoplasmic lamellar bodies.
The cells have neuroendocrine markers L-dopa decarboxylase and dense core secretory granules.
SHP-77 can serve as an in vitro target in 51Cr and 111In release cytotoxicity assays as well as in vivo nude mice assays for evaluating immune reactivity of cells and serum from lung cancer patients.
. Immune-deficient animals. Basel: Karger; 1984.
. . J. Exp. Clin. Res. 4: 355-363, 1985.
Fisher ER, et al. Comparative histopathologic, histochemical, electron microscopic and tissue culture studies of bronchial carcinoids and oat cell carcinomas of lung. Am. J. Clin. Pathol. 69: 165-172, 1978. PubMed: 204185
Koros AM, et al. Stability and utility of the unique human small cell carcinoma line SHP-77. Cancer Res. 45: 2725-2731, 1985. PubMed: 2985251
Carbone DP, et al. Neural cell adhesion molecule expression and messenger RNA splicing patterns in lung cancer cell lines are correlated with neuroendocrine phenotype and growth morphology. Cancer Res. 51: 6142-6149, 1991. PubMed: 1718595
Fisher ER, Paulson JD. A new in vitro cell line established from human large cell variant of oat cell lung cancer. Cancer Res. 38: 3830-3835, 1978. PubMed: 212181
Koros AM, et al. 186Rhenium-HNK1 monoclonal antibody targets human small cell lung cancer cells in athymic nude mice: rapid screening model for therapy. Anticancer Res. 13: 1953-1956, 1993. PubMed: 7507652
Koros. Monoclonal antibodies directed against small cell carcinomas define by flow cytometry phenotypic differences among small cell and non-small cell carcinomas, neuroblastomas, and lymphoblastoid cell lines. Lung Cancer 4: 52-54, 1988.
