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HCM-BROD-0106-C71
HCM-BROD-0106-C71
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B161434
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
HCM-BROD-0106-C71
商品貨號(hào)
B161434
Organism
Homo sapiens, human
Tissue
brain
培養(yǎng)基
Product Format
frozen 1.0 mL
Morphology
neurosphere
Culture Properties
aggregate/suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Recurrent glioblastoma
Age
See associated clinical data for patient profile information, if available.
https://portal.gdc.cancer.gov/  
https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0106-C71
Gender
See associated clinical data for patient profile information, if available.
https://portal.gdc.cancer.gov/  
https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0106-C71
Ethnicity
See associated clinical data for patient profile information, if available.
https://portal.gdc.cancer.gov/  
https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0106-C71
Applications
Basic research, compound screening.
Storage Conditions
liquid nitrogen vapor phase
Images
Clinical Data

ICD-10-CM code: C71, malignant neoplasm of cerebrum

See associated clinical data for patient profile information, if available.
https://portal.gdc.cancer.gov/  
https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0106-C71
Comments
Next-generation cancer model from the Human Cancer Models Initiate (HCMI). Refer to the following websites for additional information on this model including protocols, clinical information, and bioinformatics data.

https://ocg.cancer.gov/programs/hcmi/resources
https://portal.gdc.cancer.gov/  
https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0106-C71

Complete Growth Medium

NeuroCult NS-A Basal Medium (StemCell Technologies #05750) with NS-A Proliferation Supplement (StemCell Technologies #05754) + 20 ng/mL EGF (StemCell Technologies #78003.1) + 20 ng/mL bFGF (Peprotech #AF-100-15) + 2 µg/mL Heparin (StemCell Technologies #07980).

Prepare media according to the manufacturer’s instructions: Stem Cell Technologies Catalog #5751

Subculturing
Important: use Ultra Low Attachment (ULA) flasks/plates when culturing this model such as Corning #3814.
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2.5-5 x 105 cells/mL and maintain between below 1 x 106 cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). Large neurospheres should be mechanically dissociated to small fragments by repeated pipetting. Do not dissociate to single cells.
Cryopreservation
Complete growth media containing 10% DMSO (ATCC 4-X)
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Cells per Vial
≥ 1 x 106 cells
Volume
1.0 mL
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 9
D16S539: 9,13
D5S818: 12,13
D7S820: 9,11
THO1: 7,9.3
TPOX: 8,11
vWA: 15,16
Sterility Tests
Bacteria, yeast and fungi: No growth
Mycoplasma: No growth
Viral Testing
Hepatitis B: None detected
Cytomegalovirus: None detected
Human immunodeficiency virus: None detected
Epstein-Barr virus: None detected
Human papillomavirus: None detected
Name of Depositor
Broad Institute
Year of Origin
2018
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