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pFa6a-HIS3MX6-PADH1
pFa6a-HIS3MX6-PADH1
規(guī)格:
價(jià)格:
編號(hào):B161438
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
pFa6a-HIS3MX6-PADH1
商品貨號(hào)
B161438
Designations
pFa6a-HIS3MX6-PADH1
Depositors
DJ DeMarini
培養(yǎng)基
Biosafety Level
1
Vector Information
Construct size (kb): 4.492000102996826
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): BglII--4.4; SalI/BglII--3.6, 0.8; EcoRI--4.4.
The marker and promoter are flanked by the F4 primer site (5') and the R6 primer site (3'). The size of the F4/R6 PCR product should be 1849 nt.
F4 primer sequence: 5'-(gene-specific sequence)GAATTCGAGCTCGTTTAAAC-3'. R6 primer sequence: 5'-(gene-specific sequence) CATTGTATATGAGATAGTTGA-3'.
Contains a functional truncated (700 nt) ADH promoter. The promoter is not down regulated during the exponential phase.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

DeMarini DJ, et al. Constitutive promoter modules for PCR-based gene modification in Saccharomyces cerevisiae. Yeast 18: 723-728, 2001. PubMed: 11378899

Related Products
component of:ATCC 87819
Shipped
frozen
Shipping Information

?Freeze dried plasmid in E. coli

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