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<em>Pseudomonas aeruginosa</em> GFP
<em>Pseudomonas aeruginosa</em> GFP
規格:
價格:
編號:B161681
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Pseudomonas aeruginosa GFP
商品貨號
B161681
Verified By
Whole-genome Sequencing
Application
Fluorescence detection
Fluorescence imaging
FACS
Cell infection assays
In vivo localization
Pathogen-host interactions assays
培養基
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen
Storage Conditions
Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol&reg;
no
Type Strain
no
Antibiotic Resistance
Ampicillin resistance gene (bla) encoded on a plasmid. Confers resistance to 300 µg/mL ampicillin.
Comments
This clone was derived from ATCC® 10145™ and contains a multicopy vector encoding the green fluorescent protein GFPmut3. This gene is expressed under the control of the Plac promoter. This construct has been designed for Gram-negative bacteria fluorescence labeling.

Vector: pUCP18-MCSgfpmut3
Vector size: 5.492kb
Vector type: plasmid
Marker: bla (ampR)
Features:
GFPmut3: fluorescent maker under the control of Plac promoter
Origin of replication: ori from pRO1600
Unique restriction sites: BamHI, EcoRI, EcoRV, SmaI, XhoI
Double restriction sites: HindIII, PstI, SalI, XbaI
Medium
ATCC® Medium 2854: Nutrient Agar/Broth with 300 mcg/ml Ampicillin
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor
M Barbier
Chain of Custody
Modified by ATCC, Dr. M. Barbier <-- ATCC <-- F Kavanagh <-- Merck Sharp & Dohme
References

Barbier M and Mittar D. Novel fluorescent reporters for studying pathogen-host interactions. ASM general meeting 2014 Poster.

Choi KH, Schweizer HP. mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Nat. Protoc. 1(1): 153-161, 2006. PubMed: 17406227

Opinion 36. Designation of strain ATCC 10145 as the neotype strain of Pseudomonas aeruginosa (Schroeter) Migula. Int. J. Syst. Bacteriol. 20: 15-16, 1970.

Hugh R, Leifson E. The proposed Neotype Strains of Pseudomonas aeruginosa (Schroeter 1872) Migula 1900. Int. Bull. Bacteriol. Nomencl. Taxon 14: 69-84, 1964.

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