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LL/2-Luc2

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價格:

編號:B161692

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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甘油

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產品名稱

LL/2-Luc2

商品貨號

B161692

Organism

Mus musculus, mouse

Product Format

frozen 1.0 mL

培養基

Morphology

rounded - loosely attached or floating

Culture Properties

mixed, adherent and suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Lewis lung carcinoma

Strain

C57BL

Applications

Excellent signal/background ratio and stable luciferase expression make this cell line ideal for in vivo bioluminescence imaging of xenograft animal model to study human cancer and monitor activity of anti-cancer drug. It also can be used in cell-based assays for cancer research.

Storage Conditions

liquid nitrogen vapor phase

Tumorigenic

Yes, tested in C57BL mice

Comments

This luciferase expressing cell line was derived from parental line ATCC CRL-1642 by transduction with lentiviral vector encoding firefly luciferase gene (luc2) under control of EF-1 alpha promoter. This cell line was established through single cell cloning, and the cells constitutively express high levels of enzymatically active luciferase protein, which can be detected via in vitro and in vivo bioluminescence assays. The cells should be maintained in blasticidin (2 μg/mL) containing medium in routine cell culture. It is recommended to remove blasticidin prior to and during the experiment procedure when the cells are injected into animals in vivo, or co-cultured with other cell types in vitro.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium (DMEM; ATCC 30-2002). To make the complete growth medium, add the following components to the base medium:

Fetal bovine serum (FBS; ATCC 30-2020) to a final concentration of 10%
Blasticidin to a final concentration of 2 µg/mL

Subculturing

Subcultures are prepared by diluting the suspension 1:4 to 1:6. Cells on the floor of the flask may be dislodged by aspirating several times with culture medium or by rinsing with 0.25% trypsin - 0.53 mM EDTA solution. Corning^® T-75 flasks (catalog #430641) are recommended for subculturing this product. Cultures can be established between 4 x 10⁴ and 8 x 10⁴ viable cells/cm². Incubate cultures at 37°C.

Interval: Maintain cultures at a cell concentration between 4 X 10⁴ and 3.5 X 10⁵ cell/cm².

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Complete growth medium supplemented with 5% (v/v) DMSO (ATCC 4-X)

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

Cells per Vial

≥ 1.0 x 10⁶ cells

Volume

1.0 mL

Sterility Tests

Bacteria and yeast: No growth
Mycoplasma: No growth

Functional Tests

Luciferase activity: signal to noise ≥ 1,000 RLUs
In vitro luminesence: 10,000 photons/cell/sec, subject to imaging and culturing conditions

Population Doubling Time

approximately 13 hours

Name of Depositor

ATCC

Year of Origin

2018

References

Zinn KR, et al. Noninvasive bioluminescence imaging in small animals. ILARJ 49: 103-115, 2008. PubMed: 18172337

Dothager RS, et al. Advances in bioluminescence imaging of live animal models. Curr Opin Biotechnol 20: 45-53, 2009. PubMed: 19233638

Bertram JS, Janik P. Establishment of a cloned line of Lewis lung carcinoma cells adapted to cell culture. Cancer Lett. 11: 63-73, 1980. PubMed: 7226139

Sharma S, et al. T cell-derived IL-10 promotes lung cancer growth by suppressing both T cell and APC function. J. Immunol. 163: 5020-5028, 1999. PubMed: 10528207

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