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HCM-BROD-0036-C41

規格:

價格:

編號:B161698

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

HCM-BROD-0036-C41

商品貨號

B161698

Organism

Homo sapiens, human

Tissue

brain

培養基

Product Format

frozen 1.0 mL

Culture Properties

mixed, adherent and suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

metastatic Ewing sarcoma

Age

See associated clinical data for patient profile information, if available.
https://portal.gdc.cancer.gov/
https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0036-C41

Gender

See associated clinical data for patient profile information, if available.
https://portal.gdc.cancer.gov/
https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0036-C41

Ethnicity

See associated clinical data for patient profile information, if available.
https://portal.gdc.cancer.gov/
https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0036-C41

Applications

Basic research, compound screening.

Storage Conditions

liquid nitrogen vapor phase

Images

Clinical Data

ICD-10-CM code: C41, malignant neoplasm of bone and articular cartilage metastatic Ewing sarcoma

See associated clinical data for patient profile information, if available.
https://portal.gdc.cancer.gov/
https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0036-C41

Complete Growth Medium

Propagenix Conditioned Medium (Propagenix cat# 256-100) supplemented with 9.0 ng/mL cholera toxin (Sigma Aldrich C8052).

Prepare media according to the manufacturer’s instructions

Subculturing

Important: Pool both the adherent and suspension cell populations when passaging.

Passage cells when the culture has reached approximately 70% to 80% confluence.
Warm TrypLE (Thermofisher # 12605010) and complete growth media to room temperature.
For each flask, carefully aspirate the spent media without disturbing the monolayer.
Rinse the cell layer one time with 3 to 5 mL D-PBS (ATCC 30-2200) to remove residual medium.
Add room temperature TrypLE (1 to 2 mL for every 25 cm²) to each flask.
Gently rock each flask to ensure complete coverage of the TrypLE solution over the cells, and then aspirate the excess fluid off of the monolayer.
Observe the cells under the microscope.
When the majority of cells appear to have detached (typically 2-5 minutes), quickly add an equal volume of the complete growth medium to each flask.
Transfer the dissociated cells to a sterile centrifuge tube.
Centrifuge the cells at 200 x g for 5 minutes.
Aspirate neutralized dissociation solution from the cell pellet and resuspend the cells in 2 to 8 mL fresh, pre-warmed, complete growth medium.
Count the cells and seed new culture flasks at a density of 2 to 5 x 10⁴ viable cells per cm². Prior to seeding, aspirate the coating and discard the coating laminin solution from the vessel.
Place newly seeded flasks in a 37°C, 5% CO₂ incubator for at least 24 to 48 hours before processing the cells further.
Perform a complete medium change every 3-4 days or as needed.

Cryopreservation

Complete growth media containing 10% DMSO (ATCC 4-X)

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

Cells per Vial

≥ 1 x 10⁶ cells

Volume

1.0 mL

STR Profile

Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 12,13

D16S539: 8,11

D5S818: 11,13

D7S820: 11,13

TPOX: 8,11

TH01: 6,9

vWA: 14,17

Sterility Tests

Bacteria, yeast and fungi: No growth
Mycoplasma: No growth

Viral Testing

Hepatitis B: None detected
Cytomegalovirus: None detected
Human immunodeficiency virus: None detected
Epstein-Barr virus: None detected
Human papillomavirus: None detected

Name of Depositor

Broad Institute

Year of Origin

2018

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