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KCL22-s
KCL22-s
規(guī)格:
價(jià)格:
編號(hào):B161700
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
KCL22-s
商品貨號(hào)
B161700
Organism
Homo sapiens, human
Tissue
pleural effusion, bone marrow
培養(yǎng)基
Product Format
frozen 1.0 mL
Morphology
lymphoblast-like
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
chronic myeloid leukemia at blast crisis
Age
32 years
Gender
female
Applications
The paired KCL22-s and KCL22-r clones are useful cell line models of sensitivity and resistance to a tyrosine kinase inhibitor used in the treatment of chronic myeloid leukemia.
Storage Conditions
liquid nitrogen vapor phase
Images
Comments
BCR-ABL of the e13a2 fusion transcript type.
Complete Growth Medium

The base medium for this cell line is RPMI-1640 (ATCC 30-2001). To make the complete medium add to 500 mL of the base medium:

  • 56 mL Fetal Bovine Serum (FBS; ATCC 30-2020)
  • 5.6 mL L-glutamine (ATCC 30-2214)
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 105 cells/mL and maintain between 1.0 X 105 and 1.5 X 106 cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Complete culture medium + 10% DMSO (ATCC 4-X)
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5% CO2
Temperature: 37°C
Volume
1.0 mL
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 8
D16S539:11,12
D5S818: 10,11
D7S820: 12
THO1: 7,9
TPOX: 8
vWA: 14
Sterility Tests
Bacteria and yeast: No growth
Mycoplasma: No growth
Viral Testing
Hepatitis B: None detected
Cytomegalovirus: None detected
Human immunodeficiency virus: None detected
Epstein-Barr virus: None detected
Human papillomavirus: None detected
Population Doubling Time
24 to 30 hours
Name of Depositor
J Vaz de Melo, Dept of Hematology, Royal Adelaide Hospital, Australia
Year of Origin
1999
References

Tipping AJ, et al. Comparative gene expression profile of chronic myeloid leukemia cells innately resistant to imatinib mesylate. Exp Hematol 31(11):1073-80, 2003. PubMed: 14585372

Kubonishi I, et al. Establishment of a Ph1 chromosome-positive cell line from chronic myelogenous leukemia in blast crisis. Int J Cell Cloning 1(2):105-17, 1983. PubMed: 3461189

Kubonishi I, et al. Two new Human Myeloid Cell Lines Derived from Acute Promyelo-cytic Leukemia and Chromic myelocytic leukemia. Gan 74: 319-322 1983. PubMed: 6576961

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