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Viral vaccine production:

• Virus propagation
• Production of high-titer viral stocks
• Rescue of clinical viral isolates
• Detection of verotoxin
• Efficacy testing
• Malaria biology
• Media testing
• Mycoplasma testing
• Substrate
• Testing
• Transfection host
• Detection of virus in ground beef

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
   Cultures can be established between 2 x 10⁴ and 4 x 10⁴ viable cells/cm².
6. Incubate cultures at 37°C.

Horvath CM, Darnell JE Jr. The Antiviral State Induced by Alpha Interferon and Gamma Interferon Requires Transcriptionally Active Stat1 Protein. J Virol 70: 647-650, 1996. Pubmed: 8523587

Meraz MA, et al. Targeted disruption of the Stat1 gene in mice reveals unexpected physiologic specificity in the JAK-STAT signaling pathway. Cell 84: 431-442, 1996. Pubmed: 860859

British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 2003

Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708

American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.

Yasumura Y, Kawakita Y. Studies on SV40 in tissue culture - preliminary step for cancer research in vitro. Nihon Rinsho 21: 1201-1215, 1963.

Simizu B, et al. Characterization of the Tacaribe group of arboviruses. I. Propagation and plaque assay of Tacaribe virus in a line of African green monkey kidney cells (Vero). Proc. Soc. Exp. Biol. Med. 125: 119-123, 1967. PubMed: 6027511

Rhim JS, Schell K. Cytopathic and plaque assay of rubella virus in a line of African green monkey kiency cells (Vero). Proc. Soc. Exp. Biol. Med. 125: 602-606, 1967. PubMed: 4961492

Liebhaber H, et al. Replication of rubella virus in a continuous line of African green monkey kidney cells (Vero). Proc. Soc. Exp. Biol. Med. 125: 636-643, 1967. PubMed: 4961494

Sasaki K, et al. Studies on measles virus. II. Propagation in two established simian renal cell lines and development of a plaque assay. Kitasato Arch. Exp. Med. 37: 27-42, 1964. PubMed: 5833688

Earley E, et al. A plaque neutralization method for arboviruses. Proc. Soc. Exp. Biol. Med. 125(3): 741-747, 1967. PubMed: 15938255

Rhim JS, et al. Temperature dependence of the synthesis of adenovirus tumor and viral antigens. Proc. Soc. Exp. Biol. Med. 127: 642-646, 1968. PubMed: 5689485

Rhim JS, Schell K. Cytopathic effects of the parainfluenza virus SV5 in Vero cells. Nature 216: 271-272, 1967. PubMed: 4293683

Ozawa Y. Studies on the replication of African horse-sickness virus in two different cell line cultures. Arch. Gesamte Virusforsch. 21: 155-169, 1967. PubMed: 4232530

Rhim JS, et al. Growth of Junin virus, the etiological agent of Argentinian hemorrhagic fever, in cell cultures. Arch. Gesamte Virusforsch. 21: 243-252, 1967. PubMed: 5591575

Huber M, et al. Tyrosine phosphorylation events during coxsackievirus B3 replication. J. Virol. 71: 595-600, 1997. PubMed: 8985388

Pugachev KV, et al. Improvement of the specific infectivity of the rubella virus (RUB) infectious clone: determinants of cytopathogenicity induced by RUB map to the nonstructural proteins. J. Virol. 71: 562-568, 1997. PubMed: 8985384

Mundt W, et al. Perfusion system and a method for the large scale production of virus or virus antigen. US Patent 5,719,051 dated Feb 17 1998

Nichol PF, et al. Herpes simplex virus gene expression in neurons: viral DNA synthesis is a critical regulatory event in the branch point between the lytic and latent pathways. J. Virol. 70: 5476-5486, 1996. PubMed: 8764059

Govorkova EA, et al. African green monkey kidney (Vero) cells provide an alternative host cell system for influenza A and B viruses. J. Virol. 70: 5519-5524, 1996. PubMed: 8764064

White LJ, et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996. PubMed: 8794293

Martinez R, et al. Herpes simplex virus type 1 alkaline nuclease is required for efficient processing of viral DNA replication intermediates. J. Virol. 70: 2075-2085, 1996. PubMed: 8642627

Zeng L, et al. Identification of amino acids involved in a recognition by dengue virus NS3-specific, HLA-DR15-restricted cytotoxic CD4+ T-cell clones. J. Virol. 70: 3108-3117, 1996. PubMed: 8627790

Hill JM, et al. In vivo epinephrine reactivation of ocular herpes simplex virus type 1 in the rabbit is correlated to a 370-base-pair region located between the promoter and the 5' end of the 2.0-kilobase latency-associated transcript. J. Virol. 70: 7270-7274, 1996. PubMed: 8794381

Carter KL, et al. Characterization of the products of the UL43 gene of herpes simplex virus 1: potential implications for regulation of gene expression by antisense transcription. J. Virol. 70: 7663-7668, 1996. PubMed: 8892886

Malik AK, Weller SK. Use of transdominant mutants of the origin-binding protein (UL9) of herpes simplex virus type 1 to define functional domains. J. Virol. 70: 7859-7866, 1996. PubMed: 8892908

Chen Y, et al. Demonstration of binding of dengue virus envelope protein to target cells. J. Virol. 70: 8765-8772, 1996. PubMed: 8971005

Sandri-Goldin RM, Hibbard MK. The herpes simplex virus type 1 regulatory protein ICP27 coimmunoprecipitates with anti-sm antiserum, and the C terminus appears to be required for this interaction. J. Virol. 70: 108-118, 1996. PubMed: 8523514

Carter KL, Roizman B. The promoter and transcriptional unit of a novel herpes simplex virus 1 alpha gene are contained in, and encode a protein in frame with, the open reading frame of the alpha22 gene. J. Virol. 70: 172-178, 1996. PubMed: 8523523

Russell DW, Miller AD. Foamy virus vectors. J. Virol. 70: 217-222, 1996. PubMed: 8523528

Lukonis CJ, Weller SK. Characterization of nuclear structures in cells infected with herpes simplex virus type 1 in the absence of viral DNA replication. J. Virol. 70: 1751-1758, 1996. PubMed: 8627697

Lagunoff M, et al. Phenotypic properties of herpes simplex virus 1 containing a derepressed open reading frame P gene. J. Virol. 70: 1810-1817, 1996. PubMed: 8627705

Uprichard SL, Knipe DM. Herpes simplex ICP27 mutant viruses exhibit reduced expression of specific DNA replication genes. J. Virol. 70: 1969-1980, 1996. PubMed: 8627723

Leopardi R, Roizman B. The herpes simplex virus major regulatory protein ICP4 blocks apoptosis induced by the virus or by hyperthermia. Proc. Natl. Acad. Sci. USA 93: 9583-9587, 1996. PubMed: 8790373

Pereira ME, et al. Invasive phenotype of Trypanosoma cruzi restricted to a population expressing trans-sialidase. Infect. Immun. 64: 3884-3892, 1996. PubMed: 8751943