Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody reacts with all allotypes of mouse IgD and can be used in FACS and ELISA analysis of murine B cells.
Derivation
Lymph node cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse IgD
Cellular Products
immunoglobulin; monoclonal antibody; against mouse IgD
Comments
Animals were immunized with mouse IgD.
Lymph node cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with all allotypes of mouse IgD and can be used in FACS and ELISA analysis of murine B cells.
Complete Growth Medium
Culture Medium: RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
