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pNO1523

規格:

價格:

編號:B161717

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pNO1523

商品貨號

B161717

Designations

pNO1523

Depositors

D Dean

培養基

Biosafety Level

Vector Information

Size (kb): 5.1999998092651370

Vector: pNO1523 (plasmid)

Promoters: Promoter str

Construction: pNO1517

Marker(s):ampR

Construct size (kb): 5.199999809265137

Features: insert detection: rpsL+
marker(s): ampR
promoter: str
replicon: pMB1

Applications

vector permitting positive selection for inserts

Comments

Restriction digests of the clone give the following sizes (kb): SmaI--5.2; EcoRI--5.2.

A vector for positive selection of clones with inserts based on the dominance of the plasmid-coded rpsL+ gene.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Dean D. A plasmid cloning vector for the direct selection of strains carrying recombinant plasmids. Gene 15: 99-102, 1981. PubMed: 6271634

Shipped

freeze-dried

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