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pCMV5YQ1119
pCMV5YQ1119
規格:
價格:
編號:B161733
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pCMV5YQ1119
商品貨號
B161733
Designations
pCMV5YQ1119
GenBank Number

J02933

培養基
Species
Homo sapiens, human
Depositors
W Cheung
Applications
in another host, produces protein coagulation factor VII (serum prothrombin conversion accelerator)
Vector
Construct size (kb): 7.400000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCMV5
Intact vector size: 4.700
Type of vector: phagemid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI BamHI HindIII
Polylinker sites:
Host range: mammalian cells; Escherichia coli
Features (with orientation and position when available):
promoter: CMV, ->
terminator: hGH, ->
replicon: SV40
replicon: pMB1
marker(s): ampR, <-
replicon: f1, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: F7
Genomic copy number: unique
Gene name: coagulation factor VII (serum prothrombin conversion
accelerator)
Contains complete coding sequence?: U
Chromosome: 13; Localization: 13 q34
Genome: Homo sapiens
Gene symbol: F9
Genomic copy number: unique
Gene name: coagulation factor IX (plasma thromboplastic component,
Christmas disease, hemophilia B)
Contains complete coding sequence?: U
Chromosome: X; Localization: X q26.3-q27.1
Type of DNA: cDNA
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 2.7
Cross references: DNA Seq. Acc.: J02933
Insert lengths(kb): 2.700000047683716
Gene product: coagulation factor VII (serum prothrombin conversion accelerator) [F7]
Alleles: A1, A2, D2, C1, D1, C2
Target Gene: coagulation factor VII (serum prothrombin conversion accelerator)
Insert Size (kb)
2.700
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.7, 2.7; EcoRI--5.0, 2.5; EcoRV--7.4; PstI--4.7, 2.7; SacI--6.8, 0.5.
Digestion with EcoRI produces fragments of 5.0 and 2.4 kb.
Bacteria may grow slowly due to the high plasmid copy number.
Encodes a chimera in which aa 11-48 of processed human factor VII (Gla domain), with mutations K32E, D33N, A34T, K38T, L39E, replace the corresponding region of factor IX.
References

Andersson S, et al. Cloning, structure, and expression of a mitochondrial cytochrome P-450 sterol 26-hyroxylase, a bile acid biosnythetic enzyme. J. Biol. Chem. 264: 8222-8229, 1989. PubMed: 2722778

Cheung WF, et al. The binding of human factor IX to endothelial cells is mediated by residues 3-11. J. Biol. Chem. 267: 20529-20531, 1992. PubMed: 1400370

Toomey JR, et al. Localization of the human tissue factor recognition determinant of human factor VIIa. J. Biol. Chem. 266: 19198-19202, 1991. PubMed: 1918037

Wing-Fai Cheung, personal communication

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