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pro-caspase-3
pro-caspase-3
規格:
價格:
編號:B161776
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pro-caspase-3
商品貨號
B161776
Designations
pro-caspase-3
GenBank Number

U26943

培養基
Species
Homo sapiens, human
Depositors
G Salvesen
Applications
in another host, produces protein apopain
in another host, produces protein caspase 3, apoptosis-related cysteine protease Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease), cysteine protease CPP32
Vector
Construct size (kb): 4.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pET-23b
Intact vector size: 3.665
Type of vector: plasmid
Vector end: NdeI
Vector end: NdeI
Cloning sites: BamHI EcoRI SacI HincII SalI HindIII EagI NotI XhoI
Polylinker sites: BamHI EcoRI SacI HincII SalI HindIII EagI NotI XhoI
Other unique sites: NheI NdeI XbaI
Host range: Escherichia coli
Features (with orientation and position when available):
transcription terminator: T7, <-, 26-72
epitope tag: hexahistidine sequence (6-His), <-, 140-157
MCS: BamHI...XhoI, <-, 158-203
epitope tag: T7 tag sequence, <-, 207-239
promoter for in vitro transcription: T7, <-, 303-319
replicon: pMB1, 1450-1450
marker(s): ampR, <-, 2211-3068
replicon: f1, <-, 3200-3655
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CASP3
Other gene symbols: CPP32; CPP32B; Yama; apopain
Gene name: caspase 3, apoptosis-related cysteine protease
Contains complete coding sequence?: Y
Chromosome: 4; Localization: 4 q34
Tissue: umbilical vein endothelial cells
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 0.9
Cross references: DNA Seq. Acc.: U26943
Nucleotides ~1-900 of the insert correspond to
nucleotides ~1-834 of U26943.
Insert lengths(kb): 0.8999999761581421
Tissue: umbilical vein endothelial cells
Gene product: caspase 3, apoptosis-related cysteine protease( Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease), cysteine protease CPP32) [Yama]
Target Gene: apopain, caspase 3, apoptosis-related cysteine protease
Insert Size (kb)
0.900
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): NdeI--4.5; XbaI/XhoI--4.0, 0.95; HindIII--4.5.
Plasmid encodes the complete coding sequence, tagged at the C-terminus with hexahistidine.
References

Orth K, et al. Molecular ordering of apoptotic mammalian CED-3/ICE-like proteases. J. Biol. Chem. 271: 20977-20980, 1996. PubMed: 8702858

Quan LT, et al. Proteolytic activation of the cell death protease Yama/CPP32 by granzyme B. Proc. Natl. Acad. Sci. USA 93: 1972-1976, 1996. PubMed: 8700869

Stennicke HR, Salvesen GS. Biochemical characteristics of caspases-3, -6, -7, and -8. J. Biol. Chem. 272: 25719-25723, 1997. PubMed: 9325297

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