| Vector |
Construct size (kb): 5.199999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBS(+)
Intact vector size: 3.204
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI SalI HincII AccI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI SalI HincII AccI PstI SphI
HindIII
Construction: pUC19, f1
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CHED*
Gene name: cholinesterase-related cell division controller
Contains complete coding sequence?: Y
Tissue: glioblastoma
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.2
Cross references: DNA Seq. Acc.: M80629 Insert lengths(kb): 2.200000047683716 Tissue: glioblastoma Gene product: cholinesterase-related cell division controller [CHED*] |
| Comments |
Restriction digests of the clone give the following sizes (kb): BglII--3.5, 1.75; EcoRI--3.1, 2.2; HindIII--3.35, 1.8; PstI--3.3, 1.4, 0.46, 0.1; XbaI--5.2. The insert contains the following restriction sites (approximate kb from the 5' end): BglII--0.19, 2.0; PstI--0.35, 1.73, 1.83; HindIII--0.39; BamHI--1.88. Contains the complete coding sequence (nt 772-2028). Detects transcripts of 2.2-2.3 kb. |
