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pX65H7
pX65H7
規格:
價格:
編號:B161936
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pX65H7
商品貨號
B161936
Designations
pX65H7
Species
Homo sapiens, human
培養基
Depositors
BJ Schmeckpeper
Applications
Enzyme(s) not detecting polymorphism: EcoRI, XbaI, SstI (tested against 6 X chromosomes showing the HindIII polymorphism).
Vector
Construct size (kb): 5.150000095367432
Insert
DNA: genomic
Insert lengths(kb): 0.699999988079071
Tissue: DNA from mouse/human AHA-11a hybrid line (X only)
Gene product: DNA Segment, single copy [DXS72]
Alleles: A1, A2
Insert Size (kb)
0.700
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--4.5, 0.7; BamHI--5.1; PstI--5.1; PvuII--5.1; EcoRI--5.3; BamHI/EcoRI--4.0, 1.1.
This probe is derived from X65 (genomic lambda phage clone). This probe is tightly linked to TCD. It is deleted in XL-62 family.
DXS72 is linked to choroideremia with theta max = 0.02, LOD score = 7.09.
Linked to X-linked hypohidrotic ectodermal dysplasia (theta = 0.02, Z = 11.38).
There are no sites in the insert for BamHI, ClaI, EcoRI, EcoRV, NruI, PstI, PvuII, SalI, AvaI, BalI, NdeI, HpaI, SstI, XbaI, XhoI. There is KpnI site in the insert about 20 bp from the HindIII site nearer the vector BamHI site.
HindIII polymorphism tested in X chromosomes from Caucasians, Blacks, and cell lines. Demonstrated X-linked segregation in 3 Caucasian families.
Enzyme(s) not detecting polymorphism: EcoRI, XbaI, SstI (tested against 6 X chromosomes showing the HindIII polymorphism).
References

Schmeckpeper BJ, et al. An anonymous single-copy X-chromosome RFLP for DXS72 from Xq13-Xq22. Nucleic Acids Res. 13: 5724, 1985. PubMed: 4041033

Merry DE, et al. Choroideremia and deafness with stapes fixation: a contiguous gene deletion syndrome in Xq21. Am. J. Hum. Genet. 45: 530-540, 1989. PubMed: 2491012

Lesko JG, et al. Multipoint linkage analysis of loci in the proximal long arm of the human X chromosome: Application to mapping the choroideremia locus. Am. J. Hum. Genet. 40: 303-311, 1987. PubMed: 2883887

Zonana J, et al. X-linked hypohidrotic ectodermal dysplasia: localization within the region Xq11-21.1 by linkage analysis and implications for carrier detection and prenatal diagnosis. Am. J. Hum. Genet. 43: 75-85, 1988. PubMed: 3163892

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