美日韩一级,2021天天干,欧美少妇15p

pRLalpha2M/29J

規格:

貨期:

編號:B161972

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pRLalpha2M/29J

商品貨號

B161972

Designations

pRLalpha2M/29J

GenBank Number

J02635

Species

Rattus norvegicus, rat

Depositors

GH Fey

Vector

Construct size (kb): 7.599999904632568

Insert

DNA: cDNA

Insert lengths(kb): 4.599999904632568

Tissue: liver, male, acute phase

Gene product: alpha-2-macroglobulin [A2M]

Insert Size (kb)

4.600

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

The vector does not have convenient restriction sites close to the insert. A BamHI digest yields 3 fragments from the insert, 5' 0.7, 3.54, 0.52 kb 3'. The end fragments contain vector sequences, and the vector band is about 3 kb.

The insert contains the following restriction sites (nucleotide position according to GenBank J02635): BglII--7; BamHI--576, 4116; PvuII--637, 2174, 2536, 3040; HindIII--1440; XbaI--2217.

Restriction digests of the clone give the following sizes (kb): BamHI--3.5, 3.0, 0.6, 0.5; EcoRI--4.6, 2.6, 0.4; PstI--4.3, 2.0, 1.3; HindIII--5.6, 2.0; SalI--7.6.

Contains the complete coding sequence (nt 64-4482).

References

Gehring MR, et al. Sequence of rat liver alpha2-macroglobulin and acute phase control of its messenger RNA. J. Biol. Chem. 262: 446-454, 1987. PubMed: 2432068

Georg H Fey, personal communication

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