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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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S194/5.XXO-1
S194/5.XXO-1
規格:
貨期:
編號:B161976
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
S194/5.XXO-1
商品貨號
B161976
Organism
Mus musculus, mouse
Cell Type
B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
plasmacytoma; myeloma
Strain
BALB/c
Applications
This line was derived from an IgA secreting mineral oil induced BALB/c myeloma.
Both heavy and light chains are synthesized but not secreted.
Tested and found negative for ectromelia virus (mousepox).
Derivation
This line was derived from an IgA secreting mineral oil induced BALB/c myeloma.
Antigen Expression
H-2d; PC.1
Genes Expressed
immunoglobulin; monoclonal antibody; not secreted,H-2d; PC.1
Cellular Products
immunoglobulin; monoclonal antibody; not secreted
Comments
This line was derived from an IgA secreting mineral oil induced BALB/c myeloma.
Both heavy and light chains are synthesized but not secreted.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgA (not secreted); kappa light chain (not secreted)
Name of Depositor
R Hyman, P Ralph, S Sarkar
Deposited As
Mus musculus
References

Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881

Genuario RR, Perry RP. The GA-binding protein can serve as both an activator and repressor of ribosomal protein gene transcription. J. Biol. Chem. 271: 4388-4395, 1996. PubMed: 8626789

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