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mp35
mp35
規格:
貨期:
編號:B161990
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
mp35
商品貨號
B161990
Designations
mp35
GenBank Number

M86672

Species
Mus musculus, mouse
Depositors
U Gubler
Vector
Construct size (kb): 3.664000034332275
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK+
Intact vector size: 2.964
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I
HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI
SacII BstXI SacI
Polylinker sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I
HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI
SacII BstXI SacI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: mouse
Gene symbol: Il12a
Genomic copy number: unique
Gene name: interleukin 12A (natural killer cell stimulatory factor 1,
cytotoxic lymphocyte maturation factor 1, p35
Contains complete coding sequence?: Y
Chromosome: 3
Strain: C57BL/6
Tissue: spleen
Type of DNA: cDNA
Insert 5' end: HindIII
Insert 3' end: NotI
Insert size (kb): 0.7
Cross references: DNA Seq. Acc.: M86672
Nucleotides 54 - 687 of the insert correspond to
nucleotides 131 - 774 of M86672.
Insert lengths(kb): 0.699999988079071
Tissue: spleen
Gene product: interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35 [Il12a]
Insert Size (kb)
0.700
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): HindIII/NotI--2.9, 0.78; XbaI--2.9, 0.50, 0.28; HindIII--3.7.
The insert contains the following restriction sites (approximate kb from the 5' end): XbaI--0.49; StyI--0.40; SacI--0.68.
The sequence surrounding the initiation codon was modified to 5'-ACCAUGG-3' (Kozak consensus) to optimize translation. Thus the sequence between the vector insertion site and the initiating ATG is not derived from the mRNA.
The p35 cDNA was cloned into pBluescript SK+ via HindIII to BamHI (Aug to stop), without recreating the BamHI site.
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Schoenhaut DS, et al. Cloning and expression of murine IL-12. J. Immunol. 148: 3433-3440, 1992. PubMed: 1350290

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