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6F4C5
6F4C5
規(guī)格:
價格:
編號:B162006
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
6F4C5
商品貨號
B162006
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is directed against an antigen localized on the cytoplasmic face of the Golgi membrane.
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against rat Golgi complex (GCI)
Cellular Products
immunoglobulin; monoclonal antibody; against rat Golgi complex (GCI)
Tumorigenic
Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with a galactosyl transferase rich microsomal fraction of rat myeloma cells.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody is directed against an antigen localized on the cytoplasmic face of the Golgi membrane.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
AM Tartakoff, Y Chicheportiche
Deposited As
mouse (B cell); mouse (myeloma)
References

Chicheportiche Y, et al. Characterization of cytoplasmically oriented Golgi proteins with a monoclonal antibody. J. Cell Biol. 99: 2200-2210, 1984. PubMed: 6438114

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