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p58-CL43
p58-CL43
規(guī)格:
貨期:
編號(hào):B162017
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
p58-CL43
商品貨號(hào)
B162017
Designations
p58-CL43
GenBank Number

U24075

Species
Homo sapiens, human
Vector
Construct size (kb): 1.587000012397766
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSPORT1
Intact vector size: 4.109
Type of vector: plasmid
Vector end: SalI
Vector end: NotI
Cloning sites: AatII SphI mLuI SunI SnaBI HindIII BamHI XbaI XmaIII NotI
SpeI SstI SalI AccI SmaI EcoRI Kpn2I RsrII PinAI KpnI
Sse8387I PstI
Polylinker sites: AatII SphI mLuI SunI SnaBI HindIII BamHI XbaI XmaIII NotI
SpeI SstI SalI AccI SmaI EcoRI Kpn2I RsrII PinAI KpnI
Sse8387I PstI
Host range: Escherichia coli
Features (with orientation and position when available):
promoter for in vitro transcription: SP6, ->, 164-180
MCS: AatII...PstI, ->, 191-285
promoter for in vitro transcription: T7, <-, 298-318
insert detection: lacZ', <-, 1-341
repressor gene: lacI, <-, 464-1546
replicon: pMB1, 1903-1903
marker(s): ampR, <-, 2661-3521
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: KIR
Other gene symbols: NKB1
Gene name: killer cell inhibitory receptor
ORF's seq. position: GDB probe: GDB:698358 ()
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19 q13.4; 19, 374.860000 cR
Tissue: Resting CD3- cells
Type of DNA: cDNA
Insert 5' end: Modification: SalI linker
Insert 3' end: Modification: NotI linker
Insert size (kb): 1.587
Cross references: DNA Seq. Acc.: U24075
Nucleotides 1-1572 of the insert correspond to
nucleotides 1-1572 of U24075.
Insert lengths(kb): 1.587000012397766
Tissue: Resting CD3- cells
Gene product: killer cell inhibitory receptor [KIR]
Insert Size (kb)
1.587
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): NotI/SalI--4.4, 1.6; BglI--3.9, 1.3, 0.8; EcoRV--4.5, 1.5; PstI--5.0, 0.4, 0.35, 0.1; PvuII--3.7, 1.4, 0.6.
The insert contains the following restriction sites (approximate kb from the 5' end): BglI--1.07; EcoRV--1.00; PstI--0.06, 0.46, 0.53, 0.86; PvuII--1.15; SacI--0.47; StuI--1.07; XhoI--0.65.
Encodes a variant recognized by monoclonal antibody GL183 but not EB6.
References

Wagtmann N, et al. Molecular clones of the p58NK cell receptor reveal immunoglobulin-related molecules with diversity in both the extra- and intracellular domains. Immunity 2: 439-449, 1995. PubMed: 7749980

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