自拍偷拍第5页,欧美性大战久久久久久久蜜桃,在线观看视频日韩

CIA-E-4.15

規格:

貨期:

編號:B162028

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產品名稱

CIA-E-4.15

商品貨號

B162028

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications

The antibody reacts with human epsilon heavy chain.

Derivation

Lymph node cells were fused with S194/5.XXO.BU.1 myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against human epsilon immunoglobulin heavy chain (IgE)

Cellular Products

immunoglobulin; monoclonal antibody; against human epsilon immunoglobulin heavy chain (IgE)

Tumorigenic

Yes

Effects

Yes, forms ascites in pristane-primed BALB/c mice

(Generally, the first i.p. passage yields mostly adherent tumor cells and little ascites, but serial passage of the ascites cell in additional BALB/c mice will yield large amounts of ascites that may contain up to 20 mg/ml of the antibody. For monoclonal antibody production in ascites, cells should be injected (10 to 20 million cells) intraperitoneally (i.p.) in pristane-primed BALB/c mice.)

Comments

Animals were immunized with a human IgE paraprotein (PS).

Lymph node cells were fused with S194/5.XXO.BU.1 myeloma cells.

The antibody reacts with human epsilon heavy chain.

It has a very high affinity (3 X 10 exp-10 m/L) for an epitope located between amino acids 218 and 301 (CH2 domain) of human epsilon.

It does not bind to protein A, but functions well in histamine release assays.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Cryopreservation

Freeze medium: complete growth medium, 95%; DMSO, 5%

Culture Conditions

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Isotype

IgG1; kappa light chain

Name of Depositor

A Saxon

Deposited As

mouse (B cell); mouse (myeloma)

References

Macy E, et al. Enhanced ELISA: how to measure less than 10 picograms of a specific protein (immunoglobulin) in less than 8 hours. FASEB J. 2: 3003-3009, 1988. PubMed: 3263291

Hassner A, Saxon A. Isotype-specific human suppressor T cells for IgE synthesis activated by IgE-anti-IgE immune complexes. J. Immunol. 132: 2844-2849, 1984. PubMed: 6233363

Generally, the first i.p. passage yields mostly adherent tumor cells and little ascites, but serial passage of the ascites cell in additional BALB/c mice will yield large amounts of ascites that may contain up to 20 mg/ml of the antibody. For monoclonal antibody production in ascites, cells should be injected (10 to 20 million cells) intraperitoneally (i.p.) in pristane-primed BALB/c mice.

王經理

手機號：17280875617

QQ：1438578920
胡經理

手機號：13345964880

QQ：2438244627
周經理

手機號：17757487661

QQ：1296385441
于經理

手機號：18067160830

QQ：2088210172
沈經理

手機號：19548299266

QQ：2662369050
李經理

手機號：13626845108

QQ：972239479