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yIgH6c10
yIgH6c10
規格:
貨期:
編號:B162042
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
yIgH6c10
商品貨號
B162042
Designations
yIgH6c10
GenBank Number

Z30083

Species
Homo sapiens, human
Depositors
HC Riethman
Vector
Construct size (kb): 48.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: SuperCos 1
Intact vector size: 7.600
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI
Polylinker sites: EcoRI NotI BamHI NotI EcoRI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, neoR, G418R
promoter: T3, T7
replicon: pMB1, SV40
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D14S308
Gene name: DNA Segment, single copy
Contains complete coding sequence?: U
Chromosome: 14; Localization: 14 q32.3
Genome: Homo sapiens
Gene symbol: IGHV@
Genomic copy number: unique
Gene name: immunoglobulin heavy polypeptide, variable region (cluster)
Contains complete coding sequence?: U
Chromosome: 14; Localization: 14 q32.33
Tissue: leukocyte
Type of DNA: genomic
Insert end: Sau3AI
Insert end: Sau3AI
Insert size (kb): 40.0
Cross references: DNA Seq. Acc.: Z30083
Insert lengths(kb): 40.0
Tissue: leukocyte
Gene product: immunoglobulin heavy-chain variable region [IGHV@]
Insert Size (kb)
40.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: frozen
Comments
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Contains the 8.5 kb EcoRI fragment encoding V(H) 5-1.
Subcloned from yRM2006 (ATCC 99425). One of a set of 2 cosmid contigs ordered, from the telomeric side, yIgH6c10, 2, 4, 9, 5, 14, 3, 19, 1, and 18. The gap is between 9 and 5.
These cosmids have been assigned ATCC numbers 99460, 99452, 99456, 99458, 99472, 99462, 99454, 99466, 99450, and 99464, respectively.
References

Cook GP, et al. A map of the human immunoglobulin VH locus completed by analysis of the telomeric region of chromosome 14q. Nat. Genet. 7: 162-168, 1994. PubMed: 7920635

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