Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus diluted to provide a MOI of 0.01. Adsorb for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute the inoculum. End adsorption by adding virus growth medium.
Incubation: 1-3 days
