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pEFD53.2
pEFD53.2
規(guī)格:
貨期:
編號(hào):B162119
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pEFD53.2
商品貨號(hào)
B162119
Designations
pEFD53.2
Species
Homo sapiens, human
Applications
MspI also identifies a 2-allele RFLP (2.3, 1.9 kb) but this has not been tested by family studies.
Vector
Construct size (kb): 8.300000190734863
Insert
DNA: genomic
Insert lengths(kb): 4.699999809265137
Gene product: DNA Segment, single copy [D1S73]
Alleles: A1, A2
Insert Size (kb)
4.700
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
An EcoRI/HindIII double digest reveals fragments of 2.5, 1.7, and 1.0 kb from the insert.
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.3, 2.6, 1.5, 0.86; HindIII--4.9, 3.4; EcoRI--7.4, 0.86; PstI--5.6, 1.9, 0.74; BamHI--5.8, 2.4; consistent with deposited DNA.
Codominant segregation was demonstrated in 49 3-generation families.
MspI also identifies a 2-allele RFLP (2.3, 1.9 kb) but this has not been tested by family studies.
Enzyme(s) not detecting polymorphism: BglII, PstI, PvuII, RsaI.
References

Dracopoli NC, et al. The CEPH consortium linkage map of human chromosome 1. Genomics 9: 686-700, 1991. PubMed: 2037294

Fujimoto E, et al. Isolation and mapping of a polymorphic DNA sequence (pEFD53.2) on chromosome 1 [D1S73]. Nucleic Acids Res. 16: 3116, 1988. PubMed: 2897109

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