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pHTB [phTB]
pHTB [phTB]
規格:
貨期:
編號:B162171
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pHTB [phTB]
商品貨號
B162171
Designations
pHTB [phTB]
Species
Homo sapiens, human
Vector
Construct size (kb): 4.900000095367432
Insert
DNA: cDNA
Insert lengths(kb): 2.022000074386597
Tissue: fibroblast
Gene product: procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide (protein disulfide isomerase; thyroid hormone binding protein p55) [P4HB]
Insert Size (kb)
2.022
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): PstI/HindIII--2.7, 1.5, 0.58; HindIII--4.9; SmaI--3.2, 1.6; XbaI--4.9; PstI--4.2, 0.6.
Protein products from in vitro translation of RNA transcribed from pHTB linearized by HindIII are detected by monoclonal antibody J9. The molecular weights of these in vitro products are 57, 53, and 45 kDa.
Insert includes a PstI site at 509; AvaI sites at 133, 979, 1525, 1617, and 1637; a BamHI site at 1801, and a HindIII site at 2022.
Insert includes 5' untranslated sequences, a full coding sequence, and a truncated 3' untranslated sequence.
Hybridizes with a 2.7 kb mRNA in human A431, KB, HepG2, WI38, and rat GH3 cell lines. A minor mRNA of 4.9 kb is detected in KB, HepG2, and WI38.
Constructed by subcloning the 1.5 kb PstI/HindIII and the 0.5 kb PstI/PstI fragments from p5A5 (ATCC 59480/59481) into pGEM3.
References

Popescu NC, et al. Chromosomal localization of the gene for human thyroid hormone-binding protein. Am. J. Hum. Genet. 42: 560-564, 1988. PubMed: 2831713

Cheng SY, et al. The nucleotide sequence of a human cellular thyroid hormone binding protein present in endoplasmic reticulum. J. Biol. Chem. 262: 11221-11227, 1987. PubMed: 3611107

Sheue-Yann Cheng, personal communication

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