日屁网站,激情综合在线,黄色成人免费网站

MC3T3-E1 Subclone 14

規(guī)格:

貨期:

編號(hào):B162184

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

加入購(gòu)物車

在線咨詢收藏

產(chǎn)品名稱

MC3T3-E1 Subclone 14

商品貨號(hào)

B162184

Organism

Mus musculus, mouse

Tissue

bone, calvaria

Cell Type

preosteoblast

Product Format

frozen

Morphology

fibroblast

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age

newborn

Strain

C57BL/6

Applications

These cell lines are good models for studying in vitro osteoblast differentiation, particularly ECM signaling. They have behavior similar to primary calvarial osteoblasts.

Storage Conditions

liquid nitrogen vapor phase

Derivation

A series of subclones were isolated from the cloned but phenotypically heterogeneous MC3T3-E1 cell line.

Genes Expressed

collagen

Ref

Wang D, et al. Isolation and characterization of MC3T3-E1 preosteoblast subclones with distinct in vitro and in vivo differentiation/mineralization potential. J. Bone Miner. Res. 14: 893-903, 1999. PubMed: 10352097

Cellular Products

collagen

Ref

Tumorigenic

Yes

Effects

Yes, in immunodeficient mice

(forms bone-like ossicles)

Comments

The subclones were selected for high or low osteoblast differentiation and mineralization after growth in medium containing ascorbic acid.

The MC3T3-E1 Subclone 4 (ATCC CRL-2593) and the MC3T3 Subclone 14 (ATCC CRL-2594) lines exhibit high levels of osteoblast differentiation after growth in ascorbic acid and 3 to 4 mM inorganic phosphate.

They form a well mineralized extracellular matrix (ECM) after 10 days.

The MC3T3 Subclone 24 (ATCC CRL-2595) and the MC3T3 Subclone 30 (ATCC CRL-2596) lines exhibit poor osteoblast differentiation after growth in ascorbic acid. They do not form ECM. They can be used as negative controls for Subclones 4 and 14.Mineralizing subclones selectively express mRNAs for the osteoblast markers, bone sialoprotein (BSP), osteocalcin (OCN), and the parathyroid hormone (PTH)/parathyroid hormone-related protein (PTHrP) receptor.

Subclones with both high and low differentiation potential produce similar amounts of collagen in culture and express comparable basal levels of mRNA encoding Osf2/Cbfa1, an osteoblast-related transcription factor.

After implantation into immunodeficient mice, highly differentiating subclones form bone-like ossicles resembling woven bone, while poorly differentiating cells only produce fibrous tissue.

Complete Growth Medium

The base medium for this cell line is Alpha Minimum Essential Medium with ribonucleosides, deoxyribonucleosides, 2 mM L-glutamine and 1 mM sodium pyruvate, but without ascorbic acid (GIBCO, Custom Product, Catalog No. A1049001). To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53% (w/v) EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C

Subculture Ratio: 1:6 to 1:8

Medium Renewal: Every 2 to 3 days.

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994

Cryopreservation

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Name of Depositor

RT Franceschi

Deposited As

mouse

References

forms bone-like ossicles

B165102：MC3T3-E1 Subclone 4

B165101：MC3T3-E1 Subclone 30

B165100：MC3T3-E1 Subclone 24

王經(jīng)理

手機(jī)號(hào)：17280875617

QQ：1438578920
胡經(jīng)理

手機(jī)號(hào)：13345964880

QQ：2438244627
周經(jīng)理

手機(jī)號(hào)：17757487661

QQ：1296385441
于經(jīng)理

手機(jī)號(hào)：18067160830

QQ：2088210172
沈經(jīng)理

手機(jī)號(hào)：19548299266

QQ：2662369050
李經(jīng)理

手機(jī)號(hào)：13626845108

QQ：972239479