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MeWo
MeWo
規格:
貨期:
編號:B162209
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
MeWo
商品貨號
B162209
Organism
Homo sapiens, human
Tissue
skin, Derived from metastatic site: lymph node
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
malignant melanoma
Age
78 years
Gender
male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Images
Derivation
The MeWo cell line was initiated by Y. Kodera and M. Bean in 1974 from lymph node tissue.
Derived from metastatic site, lymph node
Clinical Data
78 years
male
White
Antigen Expression
Antigen expression: Blood type A; HLA A2, A26, Bw16, 18
Genes Expressed
Blood type A; HLA A2, A26, Bw16, 18
Tumorigenic
Yes
Effects
Yes, forms tumors in nude mice
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 12
D13S317: 8,9
D16S539: 10,12
D5S818: 12,13
D7S820: 10,12
THO1: 7,9
TPOX: 8,10
vWA: 15
Isoenzymes
ACP1, B
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 1-2
PGM3, 1
Name of Depositor
Y Kodera
Deposited As
human
Year of Origin
1974
References

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034

Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474

Carey TE, et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. PubMed: 1067619

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