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17aba
17aba
規格:
貨期:
編號:B162227
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
17aba
商品貨號
B162227
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Leukemia
Applications
The antibody reacts with human CD11b, and blocks all known CD11b dependent activities in vitro.
The antibody cross-reacts with CD11b from rat and canine cells.
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD11b
Cellular Products
immunoglobulin; monoclonal antibody; against human CD11b
Comments
Animals were immunized with human acute monocytic leukemia cells (FAM M6).
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with human CD11b, and blocks all known CD11b dependent activities in vitro.
The antibody cross-reacts with CD11b from rat and canine cells.
Complete Growth Medium
Culture Medium: Modified Dulbecco's medium (see below), 90%; Gamma
globulin-free horse serum, 10%.
DME medium with 4.5 g/L glucose 100 mL
L-Glutamine (100x) 2 mL
Solution I 1 mL
Hypoxanthine (10 mM) - Thymidine (1.6 mM) 1 mL
NCTC 135 10 mL
Gamma Globulin-Free Horse Serum (inactivated) 12 mL
Solution I (100x)
1. 1320 mg oxalacetic acid (100 mM, MW 132).
2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to
100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
aliquot and store frozen.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgM; kappa light chain
Name of Depositor
RF Todd
Deposited As
mouse (B cell); mouse (myeloma)
References

. . J. Inflamm. 13: 495-505, 1989.

Dana N, et al. Two functional domains in the phagocyte membrane glycoprotein Mo1 identified with monoclonal antibodies. J. Immunol. 137: 3259-3263, 1986. PubMed: 2430017

Mulligan MS, et al. Roles of beta 2 integrins of rat neutrophils in complement- and oxygen radical-mediated acute inflammatory injury. J. Immunol. 148: 1847-1857, 1992. PubMed: 1347308

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