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IgG-2F1
IgG-2F1
規格:
貨期:
編號:B162273
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
IgG-2F1
商品貨號
B162273
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized against GST-Rab escort protein-1 (REP-1).
The antibody is specific for REP-1 from human lymphoblastoid cell lines and peripheral blood leukocytes.
It is useful for detecting the level of REP-1. This antibody is used as a diagnostic tool for choroideremia (CHM), an X-linked form of retinal degeneration, by immunoblot analysis.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human REP-1
Cellular Products
immunoglobulin; monoclonal antibody; against human REP-1
Comments
Animals were immunized against GST-Rab escort protein-1 (REP-1).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for REP-1 from human lymphoblastoid cell lines and peripheral blood leukocytes.
It is useful for detecting the level of REP-1. This antibody is used as a diagnostic tool for choroideremia (CHM), an X-linked form of retinal degeneration, by immunoblot analysis.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cultures between 5 X 10 exp4 and 5 X 10 exp5 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1
Name of Depositor
JL Goldstein, YK Ho
Deposited As
mouse (B cell); mouse (myeloma)
References

MacDonald IM, et al. A practical diagnostic test for choroideremia. Ophthalmology 105: 1637-1640, 1998. PubMed: 9754170

Andres DA, et al. Mutational analysis of alpha-subunit of protein farnesyltransferase. Evidence for a catalytic role. J. Biol. Chem. 268: 1383-1390, 1993. PubMed: 8419339

Seabra MC, et al. Purification of component A of Rab geranylgeranyl transferase: possible identity with the choroideremia gene product. Cell 70: 1049-1057, 1992. PubMed: 1525821

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