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pACHRGcDNA [ACh receptor gamma cDNA]
pACHRGcDNA [ACh receptor gamma cDNA]
規(guī)格:
貨期:
編號:B162285
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pACHRGcDNA [ACh receptor gamma cDNA]
商品貨號
B162285
Designations
pACHRGcDNA [ACh receptor gamma cDNA]
GenBank Number

X03818

Species
Mus musculus, mouse
Depositors
JP Merlie
Vector
Construct size (kb): 4.71999979019165
Insert
DNA: cDNA
Insert lengths(kb): 1.705000042915344
Tissue: muscle BC3H-1 cell line
Gene product: acetylcholine receptor gamma [Acrg]
Insert Size (kb)
1.705
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
The insert contains the following restriction sites (nt positions according to the published sequence): HindIII--177; EcoRV--344; PvuII--554, 1070; PstI--680, 1085; AvaI--1168; BglI--1332.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.7; HindIII--3.2, 1.5; AvaI--4.1, 0.6; PstI--3.5, 1.1; BglI--2.9, 1.8.
Detects a single EcoRI fragment of 10.8 kb and a single BamHI fragment of 16.5 kb in Southern blots of genomic DNA from BC3H-1 cells.
Contains the complete coding sequence (nt 12-1571).
Detects a mRNA of 2.0 kb.
Oriented such that transcription from the SP6 promoter gives the sense strand.
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Heidmann O, et al. Chromosomal localization of muscle nicotinic acetylcholine receptor genes in the mouse. Science 234: 866-868, 1986. PubMed: 3022377

Yu L, et al. Mouse muscle nicotinic acetylcholine receptor gamma subunit: cDNA sequence and gene expression. Nucleic Acids Res. 14: 3539-3555, 1986. PubMed: 3010242

John P Merlie, personal communication

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