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pMT2-VWF
pMT2-VWF
規格:
貨期:
編號:B162294
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pMT2-VWF
商品貨號
B162294
Designations
pMT2-VWF
GenBank Number

X04385

Species
Homo sapiens, human
Depositors
Genetics Institute, Inc.
Applications
in another host, produces protein von Willebrand factor coagulation factor VIII VWF (von Willebrand factor)
Vector
Construct size (kb): 12.77499961853027
Insert
DNA: cDNA
Insert lengths(kb): 8.574999809265137
Gene product: von Willebrand factor(coagulation factor VIII VWF (von Willebrand factor)) [VWF]
Alleles: K2, M1, A4, C1, C2, D1, D2, G1, G2, K1, K2, M1, M1, M2, A1, A2, A3, A5, A6, A7, A4, C1, C2, D1, D2, G1, G2, K1, M1, M2, A1, A2, A3, A5, A6, A7, M2, M2
Target Gene: von Willebrand factor
Insert Size (kb)
8.575
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Shipped: ?Frozen E. coli containing the plasmid

Comments
The insert contains the following restriction sites (nt position according to the published sequence): SacII--1614, 1991, 7618; BglII--2240, 6935; HindIII--2334; BamHI--2816.
Restriction digests of the clone give the following sizes (kb): EcoRI--8.6, 5.5; BamHI--6.7, 3.7, 3.25, 0.5; BglII--7.0, 4.9, 2.35; HindIII--10.8, 2.5, 0.95.
Contains the complete coding sequence (nt 100 to nt 8541), with the mature peptide encoded from nt 2389 to nt 8538.
May be transfected into COS cells for transient expression of an immunoreactive product detected in conditioned medium.
The vector contains the SV40 ori, the adenovirus major late promoter and tripartite leader, the SV40 polyadenylation signal, the adenovirus VA I and VA II genes, and pBR322 sequences.
References

Bonthron DT, et al. Structure of pre-pro-von Willebrand factor and its expression in heterologous cells. Nature 324: 270-273, 1986. PubMed: 3491324

Wise RJ, et al. The propeptide of von Willebrand factor independently mediates the assembly of von Willebrand multimers. Cell 52: 229-236, 1988. PubMed: 3124962

Bonthron D, et al. Nucleotide sequence of pre-pro-von Willebrand factor cDNA. Nucleic Acids Res. 14: 7125-7127, 1986. PubMed: 3489923

D'Andrea A, et al. Recombinant DNA encoding an erythropoietin receptor. US Patent 5,278,065 dated Jan 11 1994

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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