Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
Ehlers-Danlos syndrome, type III
Age
23 years
Gender
male
Clinical Data
The tissue donor suffered from type III Ehlers-Danlos syndrome, and was the son of Ad Hot (ATCC CRL-1227) who was affected with the same condition.
male
Comments
The tissue donor suffered from type III Ehlers-Danlos syndrome, and was the son of Ad Hot (ATCC CRL-1227) who was affected with the same condition.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days.
