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J27-neo
J27-neo
規格:
貨期:
編號:B162355
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
J27-neo
商品貨號
B162355
Organism
Mus musculus, mouse
Cell Type
fibroblast
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain
C3H
Applications
J27-neo is a stable transfected cell line established in 1987 by transfection by calcium phosphate of the J27.2 cell line with a modified neomycin drug-resistant eukaryotic vector, pSP65-Neo.
J27-neo does not secrete or express HLA B7.
Derivation
J27-neo is a stable transfected cell line established in 1987 by transfection by calcium phosphate of the J27.2 cell line with a modified neomycin drug-resistant eukaryotic vector, pSP65-Neo. The cells were selected in HAT and G418.
Comments
J27-neo is a stable transfected cell line established in 1987 by transfection by calcium phosphate of the J27.2 cell line with a modified neomycin drug-resistant eukaryotic vector, pSP65-Neo. The cells were selected in HAT and G418.
The vector did not carry an insert.
J27-neo does not secrete or express HLA B7.
J27.2 is a C3H mouse fibroblast L cell line stably expressing the tk and human b2m genomic genes.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor
F Grumet
Year of Origin
1987
References

Kavathas P, Herzenberg LA. Stable transformation of mouse L cells for human membrane T-cell differentiation antigens, HLA and beta 2-microglobulin: selection by fluorescence-activated cell sorting. Proc. Natl. Acad. Sci. USA 80: 524-528, 1983. PubMed: 6188154

Hiraki DD, et al. Bioengineered soluble HLA-B7. Genesis, characterization, and occurrence of dimerization. Hum. Immunol. 40: 235-246, 1994. PubMed: 7960968

Cohen N, et al. Secretion of genetically engineered human/mouse class I antigens. Hum. Immunol. 25: 207-222, 1989. PubMed: 2670852

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