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lambdaSH3 [pcD-GALT]
lambdaSH3 [pcD-GALT]
規格:
貨期:
編號:B162371
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
lambdaSH3 [pcD-GALT]
商品貨號
B162371
Designations
lambdaSH3 [pcD-GALT]
GenBank Number

M13430

Species
Homo sapiens, human
Vector
Construct size (kb): 48.29999923706055
Insert
DNA: genomic
Insert lengths(kb): 18.79999923706055
Tissue: B lymphocyte (EBV transformed), SH cell line
Gene product: T cell receptor, gamma, variable region V3 [TCRGV1]
Insert Size (kb)
18.800
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): KpnI--17.5, 10.0 (doublet), 4.9, 3.9, 1.5; PvuI--23.0, 13.0, 8.0; SacI--20.0, 9.0, 6.0, 4.7, 4.4, 1.5, 1.1; XbaI--20.0, 19.0, 9.0; XhoI--20.0, 16.0, 9.0, 2.3.
The insert contains the following restriction sites (kb from the 5' end): SacI--0.6, 0.8, 7.0, 8.6, 13.1, 17.7, 18.8 (linker).
Contains the genes in the germ line configuration, beginning about 9 kb upstream of pseudogene Vgamma1 and ending just after Vgamma3.
The insert extends 5.6 kb further in the 5' direction and is 1.3 kb shorter at the 3' end than the insert in lambdaSH2, and extends 1.3 kb further in the 5' and is 0.2 kb shorter at the 3' end than lambdaSH4.
Additional GenBank/EMBL accession numbers: M12949, M13429.
References

LeFranc MP, et al. Diversity and rearrangement of the human T cell rearranging gamma genes: nine germ-line variable genes belonging to two subgroups. Cell 45: 237-246, 1986. PubMed: 2938743

Marie-Paule Lefranc, personal communication

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