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pSL7
pSL7
規(guī)格:
貨期:
編號(hào):B162372
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pSL7
商品貨號(hào)
B162372
Designations
pSL7
Depositors
T Lufkin
Biosafety Level
1
Vector Information
Size (kb): 6.4000000953674320
Vector: pSL7 (plasmid)
Construction: pBS
Marker(s):ampR
Construct size (kb): 6.400000095367432
Features: marker(s): ampR
other: internal ribosome entry sequence (IRES)
replicon: pMB1
reporter group: alkaline phosphatase
terminator: SV40 polyadenylation
Applications
reporter construct alkaline phosphatase, placental (Regan isozyme)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.1, 2.5, 0.6, 0.2; HindIII--5.5, 0.6, 0.3; SpeI--6.4.
Plasmid expressing alkaline phosphatase. Foreign genes can be cloned upstream of the IRES/reporter gene, allowing production of a dicistronic messenger RNA in mammalian cells.
IRES/reporter insert lacking polyA can be excised with NruI.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Li X, et al. Dicistronic lacZ and alkaline phosphatase reporter constructs permit simultaneous histological analysis of expression from multiple transgenes. BioTechniques 23: 874-882, 1997. PubMed: 9383553

Thomas Lufkin, personal communication

Shipped
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