黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車(chē) 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > pSCH2003 plasmid in E. coli B162377
pSCH2003 plasmid in E. coli
pSCH2003 plasmid in E. coli
規(guī)格:
貨期:
編號(hào):B162377
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
pSCH2003 plasmid in E. coli
商品貨號(hào)
B162377
Designations
pSCH2003 plasmid in E. coli
GenBank Number

X04555

Species
Unidentified bacterium
Depositors
KJ Shaw
Vector
Construct size (kb): 3.25
Insert
DNA: Synthetic
Insert lengths(kb): 0.305
Gene product: aminoglycoside adenylyltransferase(spectinomycin resistance, streptomycin resistance) [ant(2")-Ia]
Insert Size (kb)
0.305
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): XhoI--3.3; HindIII--3.3; PstI--3.3; HindIII/XhoI--2.8, 0.4.
The insert contains the following restriction sites (approximate kb from the 5' end): PvuII--0.20; RsaI--0.25; SphI--0.29.
A single gel purification of the PCR generated probe is necessary since flanking regions will co-amplify with the gene specific sequence. Failure to do so often results in high backgrounds and false positives with clinical E. coli strains.
A hybridization probe may be generated using the following vector specific PCR primers: modified T3 = 5'-CCCCTCACTAAAGGGAACAAAAGCTG-3' and modified T7 = 5'-CGCGTAATACGACTCACTATAGGGCGAA-3'.
References

Shaw KJ, et alThe application of molecular techniques for the study of aminoglycoside resistanceIn: Shaw KJ, et alMethods in molecular medicine: molecular approaches for the diagnosis and investigation of bacterial diseasesTotowa, NJHumana Presssubmitted, 1996

  • 王經(jīng)理
    手機(jī)號(hào)：17280875617
    QQ：1438578920
  • 胡經(jīng)理
    手機(jī)號(hào)：13345964880
    QQ：2438244627
  • 周經(jīng)理
    手機(jī)號(hào)：17757487661
    QQ：1296385441
  • 于經(jīng)理
    手機(jī)號(hào)：18067160830
    QQ：2088210172
  • 沈經(jīng)理
    手機(jī)號(hào)：19548299266
    QQ：2662369050
  • 李經(jīng)理
    手機(jī)號(hào)：13626845108
    QQ：972239479