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13762 MAT B III
13762 MAT B III
規(guī)格:
貨期:
編號(hào):B162378
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
13762 MAT B III
商品貨號(hào)
B162378
Organism
Rattus norvegicus, rat
Tissue
mammary gland
Product Format
frozen
Morphology
epithelial
Culture Properties
suspension or loosely adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
adenocarcinoma
Strain
Fischer 344 (F344)
Storage Conditions
liquid nitrogen vapor phase
Derivation
The 13762 MAT B III cell line was established at the EG&G Mason Research Institute from a transplantable rat ascites tumor derived from the 13762 solid mammary adenocarcinoma.
Comments
Prior to its deposit, the cell line was tested and found negative for the following viruses:
Pneumonia virus of mice (PVM), reovirus type 3 (Reo3), K virus, polyomavirus, Sendai virus, GDVII (mouse poliovirus), Kilham rat virus (KRV), minute virus of mice (MVM), SV5, H-1 virus and ectromelia virus.
The line grows as small clusters of rounded cells lightly attached to the substrate, or as a static suspension.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 x 105 viable cells/mL and maintain at 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Name of Depositor
AE Bogden
Deposited As
Rattus sp.
References

Xing RH, et al. Prevention of breast cancer growth, invasion, and metastasis by antiestrogen tamoxifen alone or in combination with urokinase inhibitor B-428. Cancer Res. 57: 3585-3593, 1997. PubMed: 9270032

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