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1. Remove and discard medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually 5 to 10 min).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detatch. Cells that are difficult to detatch may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.

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Nucleotide (GenBank) : AF093020 Chlorocebus aethiops clone M9 22k48 RNA, primary transcript.

Nucleotide (GenBank) : AF093019 Chlorocebus aethiops clone M10 22k48 RNA, primary transcript.