毛片网站视频,亚洲国产第一,亚洲四区

SIRC [Statens Seruminstitut Rabbit Cornea]

規格:

貨期:

編號:B162402

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產品名稱

SIRC [Statens Seruminstitut Rabbit Cornea]

商品貨號

B162402

Organism

Oryctolagus cuniculus, rabbit

Tissue

cornea

Product Format

frozen

Morphology

fibroblast

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications

This cell line is suitable for primary isolation of rubella virus. The early appearance of distinct cytopathic changes makes this cell line highly suitable for both the propagation and quantitation of rubella virus.

Storage Conditions

liquid nitrogen vapor phase

Karyotype

modal number = 66; range = 51 to 90
The modal number (66) does not permit sexing of donor cytologically. Most cells contain one long, unpaired, submetacentric chromosome and 2 to 3 small telocentric chromosomes with satellites. Polyploidy was observed in 5/200 metaphase cells.

Derivation

The SIRC cell line was derived by M. Volkert of the Staatens Seruminstitut, Copenhagen, Denmark, from the cornea of a normal rabbit in 1957.

Virus Susceptibility

Rubella virus , Rubella virus

Comments

In 1965, J. Leerhoy found this line to be susceptible to rubella virus. C.A. Phillips, et al. confirmed the above findings and demonstrated the suitability of the cell line for primary isolation of rubella virus.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: Twice per week

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

Name of Depositor

J Leerhoy

Deposited As

Oryctolagus cuniculus

Passage History

Little is recorded relating to the history of this cell line for approximately the first 400 passages.

Year of Origin

1957

References

Leerhoy J. Cytopathic effect of rubella virus in a rabbit-cornea cell line. Science 149: 633-634, 1965. PubMed: 14331182

Phillips CA, et al. Isolation, propagation and neutralization of rubella virus in cultures of rabbit cornea (SIRC) cells. Proc. Soc. Exp. Biol. Med. 122: 783-786, 1966. PubMed: 5918951

Rhim JS, et al. Plaque assays of rubella virus in cultures of rabbit cornea (SIRC) cells. Proc. Soc. Exp. Biol. Med. 125: 1271-1274, 1967. PubMed: 6042441

Farris AD, et al. Conserved features of Y RNAs revealed by automated phylogenetic secondary structure analysis. Nucleic Acids Res. 27: 1070-1078, 1999. PubMed: 9927741

王經理

手機號：17280875617

QQ：1438578920
胡經理

手機號：13345964880

QQ：2438244627
周經理

手機號：17757487661

QQ：1296385441
于經理

手機號：18067160830

QQ：2088210172
沈經理

手機號：19548299266

QQ：2662369050
李經理

手機號：13626845108

QQ：972239479