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5HT1Dalpha
5HT1Dalpha
規(guī)格:
貨期:
編號:B162413
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
5HT1Dalpha
商品貨號
B162413
Designations
5HT1Dalpha
Species
Homo sapiens, human
Depositors
MJ Bienkowski, SA Veldman
Applications
in another host, produces protein 5-hydroxytryptamine (serotonin) receptor 1D alpha
Vector
Construct size (kb): 9.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: p3CLHygro
Intact vector size: 8.300
Type of vector: plasmid
Vector end: SalI
Vector end: SalI
Cloning sites: HindIII SalI
Polylinker sites: HindIII SalI
Construction: p3CL, pHEBo (hygR)
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, hygR
promoter: CMV
replicon: pMB1, SV40
terminator: bGH polyadenylation
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: HTR1DA*
Gene name: 5-hydroxytryptamine (serotonin) receptor 1D alpha
Contains complete coding sequence?: Y
Type of DNA: genomic
Insert end: SalI
Insert end: SalI
Insert size (kb): 1.1
Cross references:
Insert lengths(kb): 1.100000023841858
Gene product: 5-hydroxytryptamine (serotonin) receptor 1D alpha [HTR1DA*]
Target Gene: 5-hydroxytryptamine (serotonin) receptor 1D alpha
Insert Size (kb)
1.100
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--8.6, 0.92; BglII--7.8, 1.2, 0.48; HindIII--8.3, 0.92, 0.29; SalI--8.3, 1.2; XhoI--5.2, 4.3; EcoRI--6.0, 2.4, 1.0.
Insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.09, 1.00; BglII--0.11, 0.58; HindIII--0.84, 1.10; XhoI--0.88.
Contains the complete coding sequence with only a few extra nucleotides.
The order of the major features of the plasmid is: ampR - EcoRI - SV40 ori - SV40 promoter - XhoI - hygR - tk polyadenylation - TaqI - SV40 polyadenylation - EcoRI - CMV promoter >- HindIII - SalI - insert >- SalI - bGH polyadenylation - PvuI.
References

Sarah A Veldman, personal communication

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