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pCMV4
pCMV4
規格:
貨期:
編號:B162456
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pCMV4
商品貨號
B162456
Designations
pCMV4
Depositors
DL Davis
Biosafety Level
1
Vector Information
Size (kb): 4.8740000724792480
Vector: pCMV4 (phagemid)
Promoters: Promoter CMV
Construction: pTZ18R, bacteriophage f1, CMV promoter
Marker(s):ampR
Construct size (kb): 4.874000072479248
Features: marker(s): ampR
promoter: CMV
replicon: f1
MCS: BglI...SmaI
transcription terminator: hGH
enhancer: SV40
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.6, 1.3; BglII--4.9; BglII/SmaI--4.9.
Should be grown in media containing ampicillin at a final concentration of 1 mg/ml to improve plasmid yields.
May be used in essentially all cultured cells in transient transfection assays, to establish permanent cell lines and to construct transgenic mice.
This vector contains a translational enhancer in the sequence prior to the polylinker.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Andersson S, et al. Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme. J. Biol. Chem. 264: 8222-8229, 1989. PubMed: 2722778

Shipped
freeze-dried
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