Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.8, 0.80, 0.70; BamHI--4.4; BglII--4.4; EcoRI--3.5, 0.86; HindIII--4.4.
The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.28; BglII--0.47, 0.5; EcoRI--0.75; AccI--1.12. The insert is not cut with HindIII, BamHI, XbaI, SalI, or HincII.
Detects 9 exons contained in human genomic EcoRI fragments of the following sizes (kb, 5' to 3'): 7.5 (contains 3 exons), 4.5 or 4.2, 12.5, 2.6, 4.0, 1.0, 7.0.
Corresponds to the 5' EcoRI/NcoI fragment of T8.
Transcription from the SP6 and T7 promoters give the sense and antisense strands, respectively.