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puc19TM15 [lambda HTm15]
puc19TM15 [lambda HTm15]
規(guī)格:
貨期:
編號:B162518
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱
puc19TM15 [lambda HTm15]
商品貨號
B162518
Designations
puc19TM15 [lambda HTm15]
GenBank Number

M16552

Species
Homo sapiens, human
Vector
Construct size (kb): 6.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 3.700000047683716
Tissue: umbilical vein endothelial cells
Gene product: thrombomodulin [THBD]
Insert Size (kb)
3.700
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
The insert contains the following sites separated by (bp)(approx): EcoRI- 270- SmaI- 160- PstI- 240- PstI- 210- PstI- 100- PstI- 530- KpnI- 310- PstI- 1080- HindIII- 110- BamHI- 205- PstI- 475- EcoRI.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 2.7; SacI--6.5; AvaI--3.4, 2.0, 0.2; XbaI--5.9, 0.54, 0.2, 0.1.
The insert includes 146 nt of 5'-noncoding sequence, an open reading frame of 1725 nt and 1779 nt of 3'-noncoding sequence including 40 nt of poly(A) tail.
There is 64% homology between this probe and bovine thrombomodulin.
A single band of 3.7 kb is detected in human placenta and endothelial cell poly(A)+ RNA.
References

Wen DZ, et al. Human thrombomodulin: complete cDNA sequence and chromosome localization of the gene. Biochemistry 26: 4350-4357, 1987. PubMed: 2822087

Espinosa R, et al. Regional localization of the human thrombomodulin gene to 20p12-cen. Genomics 5: 649-650, 1989. PubMed: 2559025

Scarpati EM, Sadler JE. Regulation of endothelial cell coagulant properties. J. Biol. Chem. 264: 20705-20713, 1989. PubMed: 2555368

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