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RG7/7.6 HL
RG7/7.6 HL
規格:
貨期:
編號:B162564
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
RG7/7.6 HL
商品貨號
B162564
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue
spleen
Cell Type
hybridoma: B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions
liquid nitrogen vapor phase
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against rat kappa light chain [kappa 1b (LEW)]
Cellular Products
immunoglobulin; monoclonal antibody; against rat kappa light chain [kappa 1b (LEW)]
Comments
Animals were immunized with pooled rat IgG.
Spleen cells were fused with NS-1 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
mouse IgG2a
Name of Depositor
TA Springer
Deposited As
mouse (B cell); mouse (myeloma)
References

Springer TA, et al. Monoclonal antibodies specific for rat IgG1, IgG2a and IgG2b subclasses, and kappa chain monotypic and allotypic determinants: reagents for use with rat monoclonal antibodies. Hybridoma 1: 257-273, 1982. PubMed: 6208128

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