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UCD/PR 10.11
UCD/PR 10.11
規(guī)格:
貨期:
編號(hào):B162577
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
UCD/PR 10.11
商品貨號(hào)
B162577
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain
BALB/c (B cell); BALB/c (myeloma)
Applications
The antibody reacts type 8 and type 18 cytokeratins in immunoblots.
The antibody can be used to detect cytokeratin in formalin or B-5 fixed paraffin sections.
The antibody primarily reacts with protein antigens in ductal portions of glands and with primary and metastatic adenocarcinomas.
Radioactively labeled UCD/PR 10.11 antibody can also be used to detect soluble extracellular cytokeratin in a quantitative sandwich radioimmunoassay.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with soluble extracellular cytokeratin.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against type 8 and type 18 cytokeratins
Cellular Products
immunoglobulin; monoclonal antibody; against type 8 and type 18 cytokeratins
Comments
The antibody reacts type 8 and type 18 cytokeratins in immunoblots.
The antibody can be used to detect cytokeratin in formalin or B-5 fixed paraffin sections.
The antibody primarily reacts with protein antigens in ductal portions of glands and with primary and metastatic adenocarcinomas.
Radioactively labeled UCD/PR 10.11 antibody can also be used to detect soluble extracellular cytokeratin in a quantitative sandwich radioimmunoassay.
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
    • Subculturing
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
    Medium Renewal: Every 2 to 3 days
    Cryopreservation
    culture medium 95%; DMSO, 5%
    Culture Conditions
    Temperature: 37°C
    Isotype
    IgG
    Name of Depositor
    Regents of the University of California
    Deposited As
    mouse (B cell); mouse (myeloma)
    U.S. Patent Number
    References

    Cardiff RD, et al. Cytokeratin tumor markers and assays for their detection. US Patent 4,775,620 dated Oct 4 1988

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