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當(dāng)前位置: 首頁 > ATCC代理 > HFBDN02 B162614
HFBDN02
HFBDN02
規(guī)格:
貨期:
編號(hào):B162614
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
HFBDN02
商品貨號(hào)
B162614
Designations
HFBDN02
GenBank Number

T06019

Species
Homo sapiens, human
Vector
Construct size (kb): 5.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GOT2
Genomic copy number: unique
Gene name: glutamic-oxaloacetic transaminase 2, mitochondrial
(aspartate aminotransferase 2)
Contains complete coding sequence?: N
Chromosome: 16; Localization: 16 q13
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.0
Cross references: DNA Seq. Acc.: M22632; EST: EST03908
Nucleotides 85-303 of the insert correspond to
nucleotides 2137-1919 of M22632 with 97% identity.
DNA Seq. Acc.: T06019; EST: EST03908; Primer: M13-21
Insert lengths(kb): 2.0
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)( Glutamate oxaloacetate transaminase 2, mitochondrial (aspartate aminotransferase 2), mitochondrial aspartate aminotransferase) [GOT2]
Insert Size (kb)
2.0
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.0.
Based on the alignment with GenBank M22632 (2339 nt, CDS 9-1301) and the insert size, this clone probably contains some of the coding sequence and most of the 3' untranslated region.
EST03908 contains a Line 1 element: 1-85.
References

Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434

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